pi cell cycle detection kit  (Keygen Biotech)

Journal: RSC Advances

Article Title: β-caryolane derivatives as novel anti-colorectal cancer agents: synthesis and in vitro biological evaluation

doi: 10.1039/d5ra07312j

Figure Lengend Snippet: NC-19 induces HT-29 G0/G1 phase cell cycle arrest and ROS generation. (A and B) Cell cycle analysis of HT-29 cell cycle distribution after treatment with NC-19 (0, 2, 4, 8 µM) for 24 hours. (C and D) Western blot and bar graph analysis of CDK2 and Cyclin E protein expression in HT-29 cells after treatment with NC-19 (0, 5, 10, 20 µM) for 24 hours. (E) Cell viability of HT-29 cells pretreated with NAC (2 mM) for 8 hours, followed by incubation with or without 2 µM NC-19 for 72 hours. (F) Detection of ROS generation by DCFH-DA in HT-29 cells after treatment with NC-19 (0, 1, 2, 5, 10 µM) for 24 hours.

Article Snippet: NAC, DCFH-DA, RIPA lysis buffer, protease inhibitors, phosphatase inhibitors, 4% paraformaldehyde fix solution, hematoxylin and eosin staining kit, Western blocking buffer, Enhanced BCA protein assay kit, SDS-PAGE gel kit, BeyoECL Plus kit, Annexin V-FITC cell apoptosis detection kit and PI cell cycle detection kit were purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: Cell Cycle Assay, Western Blot, Expressing, Incubation

Journal: Frontiers in Oncology

Article Title: CIK-augmented anti-PD1/CTLA4 immunotherapy eradicates chemo-resistant ovarian cancer via tripartite mechanistic synergy

doi: 10.3389/fonc.2025.1670033

Figure Lengend Snippet: Treatment effects on ovarian cancer cell apoptosis. (A-F) Annexin V-FITC/PI flow cytometry scatter plots of (A/C/E) A2780 and (B/D/F) SKOV3 cells under control, N+I, and N+I+CIK treatments, respectively. (G/H) Corresponding quantitative analysis of apoptosis rates for (G) A2780 and (H) SKOV3 cells under the indicated treatments. n=3, ***=0.0009, ***=0.0001, ***=0.0001, ***=0.0001 vs respective control groups.

Article Snippet: Immune checkpoint inhibitors Nivolumab (HY-P9903, MCE) and Ipilimumab (HY-P9901, MCE); apoptosis and cell cycle detection kits Annexin V-FITC/PI (CA1020, Solarbio) and DNA Content Assay Kit (CA1510, Solarbio); extracellular matrix component Matrigel (356234, Corning); cell viability reagent CCK-8 Kit (CK04, Dojindo); staining agent Crystal Violet (C8470, Solarbio); and fixation solution 4% Paraformaldehyde (G1113, Servicebio).

Techniques: Flow Cytometry, Control